FastQCFastQC Report
Thu 2 Jun 2016
DRR025003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650065
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108700.29780291583848506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97850.268077417799409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.1667641535150744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.056.8655321
GTATCAA118250.041.1295551
ATCAACG149250.032.31913
TCAACGC150250.032.002674
TATCAAC154600.031.231952
CAACGCA155050.030.9816275
GTGGTAT26050.030.5150151
AACGCAG161400.029.90756
TGGTATC28300.026.4148922
ACGCAGA184850.025.8807017
CGCAGAG183800.025.8240058
GTACATG92600.023.8737951
GCAGAGT198300.023.6039319
ACAACGC6100.023.121593
TATAACG2905.456968E-1222.6969872
TACATGG93750.022.366692
TAGACCG2206.312257E-821.3649755
AGAGTAC187300.021.21404810-11
ACATGGG94650.020.812283
GAGTACT151150.020.55138212-13
Produced by FastQC (version 0.11.5)