FastQCFastQC Report
Thu 2 Jun 2016
DRR025002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347668
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90420.27009846854586533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.22275207696820593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44450.13277899720043923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.056.338031
GTATCAA99750.041.481241
ATCAACG124550.032.917653
TCAACGC126100.032.6224484
TATCAAC129900.031.4176042
CAACGCA132950.031.116125
TACCTGG26400.029.5642362
AACGCAG140550.029.56256
GTGGTAT24600.027.7149981
GTCTCGC10000.027.2715591
GTACCTG30150.026.668021
ACGCAGA155150.026.4768567
CGCAGAG154550.026.366378
TCGCTCT11600.024.7226734
TGGTATC24750.024.696232
TCTGTCG11550.024.415728
ACAACGC6950.024.354193
GCAGAGT162950.024.1411749
GTACATG75650.023.9295081
TACAACG6950.023.678042
Produced by FastQC (version 0.11.5)