FastQCFastQC Report
Thu 2 Jun 2016
DRR025002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347668
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101410.30292729147573777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87980.2628098126815443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58440.1745692822585752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61350.056.0431821
GTATCAA115200.040.216451
TATAACG3500.033.5822832
ATCAACG141400.032.715833
TCAACGC144950.031.843534
ATAACGC3550.031.7830223
TATCAAC147100.031.4500032
CAACGCA149850.030.9277345
AACGCAG156750.029.5963086
ACAACGC4800.028.4033163
TAACGCA4650.026.2813914
ACGCAGA175600.026.174447
CGCAGAG174550.026.170358
TCTGTCG11850.025.3819358
GTGGTAT28550.024.7118431
TACCTGG27100.024.461782
TGGTATC27400.024.365542
GTACCTG28200.024.3513891
GTCTCGC10600.023.9611631
GCAGAGT187800.023.5982199
Produced by FastQC (version 0.11.5)