FastQCFastQC Report
Thu 2 Jun 2016
DRR025001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414480
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105610.2392354252369475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86800.19662565013319802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.12277776770990015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89000.041.7358781
GTATCAA151950.037.1324041
ATCAACG182900.030.4355373
TCAACGC183650.030.3603784
TATCAAC189050.029.6706052
CAACGCA190800.029.245315
AACGCAG198650.028.2034826
CGCAGAG217650.025.1790988
ACGCAGA220050.025.1394257
GTACATG131850.025.0696161
TACATGG127750.024.1069932
GCAGAGT234450.022.8335679
ACATGGG132050.022.217193
AGAGTAC200800.021.03062410-11
GAGTACT142500.020.76045612-13
GTACCTG33800.019.8926121
AGTACTT152250.019.53900312-13
TACCTGG32500.019.0964762
CAGAGTA233750.018.58887510-11
GTACTTT159200.018.56919714-15
Produced by FastQC (version 0.11.5)