FastQCFastQC Report
Thu 2 Jun 2016
DRR025001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414480
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.27244431960276183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99120.22453380692629712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70320.15929395987749406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97300.043.6591641
GTATCAA166650.036.9517361
ATCAACG210250.028.9560243
TCAACGC212250.028.6542054
TATCAAC217300.028.0184882
CAACGCA218800.027.7319725
AACGCAG227400.026.765256
ACAACGC6800.023.5058083
ACGCAGA257550.023.48447
CGCAGAG255800.023.3873238
GTACCGG3100.022.763031
GTACATG129300.022.7031731
TATAACG4600.022.4853442
TACATGG126050.021.6331522
ATAACGC5500.021.3689163
AGAGTAC236650.021.34787210-11
GCAGAGT273750.021.2872819
TATACCG3401.8189894E-1220.7354855
ACATGGG128200.020.4621523
AGTACTT181800.020.27924212-13
Produced by FastQC (version 0.11.5)