FastQCFastQC Report
Thu 2 Jun 2016
DRR025000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528231
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113700.25109143062710365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96460.21301916797089193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.1273565769944157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71100.055.159211
GTATCAA133750.043.1743851
ATCAACG162050.035.1973043
TCAACGC162900.035.097914
CAACGCA172800.033.084175
TATCAAC174900.032.665832
AACGCAG181400.031.612026
GTGGTAT30850.029.8760051
ACGCAGA203150.027.9011337
CGCAGAG203800.027.4886768
GTACATG147450.027.3311311
TGGTATC31750.027.1028272
TACATGG143150.026.278822
GCAGAGT216100.025.141139
ACATGGG146250.024.9495933
GAGTACT147900.021.03454812-13
CAGAGTA213000.020.91753410-11
GAGTACA108150.020.8706671
CATGGGG82650.020.7643724
AGAGTAC203350.020.65057410-11
Produced by FastQC (version 0.11.5)