FastQCFastQC Report
Thu 2 Jun 2016
DRR025000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528231
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130310.287772421504115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115030.2540285599387487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.16478841295861452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77200.053.9235041
GTATCAA143700.042.0300981
ATCAACG179850.033.1695373
TCAACGC182250.032.724784
TATCAAC190550.031.4820882
CAACGCA190500.031.4062545
AACGCAG197500.030.3403786
GTGGTAT36950.028.7704141
TGGTATC36700.026.7733082
GTACATG146650.026.4941581
ACGCAGA226750.026.197427
CGCAGAG229950.025.710238
TACATGG143350.025.2531072
ACATGGG145650.024.0132353
GCAGAGT244450.023.5315279
AGAGTAC232100.020.31893510-11
GAGTACT172150.019.91484812-13
AGTACTT176700.019.69460312-13
CAGAGTA242100.019.67377510-11
CATGGGG81600.019.179484
Produced by FastQC (version 0.11.5)