FastQCFastQC Report
Thu 2 Jun 2016
DRR024999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279377
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80510.24550394785351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69000.21040581793432106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.1295367992152168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53450.048.9049341
GTATCAA94000.040.261841
ATCAACG114150.032.3290443
TACCTGG28600.032.2187772
TCAACGC115050.032.1568874
CAACGCA117800.031.3634175
TATCAAC119050.031.0788062
AACGCAG123050.030.2908336
ACGCAGA139250.026.3947727
GTGGTAT22950.025.8115121
CGCAGAG144000.025.4258068
GTACCTG36600.025.3052561
GTACATG105750.024.8072971
TACATGG104350.023.7881372
TATAACG3000.023.5065562
GCAGAGT154550.023.1728329
ACCTGGG39350.022.6990643
ACATGGG106200.022.6644153
TGGTATC27100.021.6850152
ATAACGC4050.020.893763
Produced by FastQC (version 0.11.5)