FastQCFastQC Report
Thu 2 Jun 2016
DRR024999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279377
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93550.28526759808341645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80110.24428420398142697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53930.16445196755359326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.053.106721
GTATCAA102100.039.5279851
ATCAACG128950.030.5132753
TACCTGG27300.030.4804842
TCAACGC130300.030.2281444
TATCAAC133750.029.771512
CAACGCA133000.029.614495
AACGCAG137750.028.6269866
GTGGTAT25950.027.7330231
TATAACG3200.024.975272
GTACCTG35400.024.847431
GTACATG105100.024.7494491
ACGCAGA159250.024.582417
TGGTATC28500.024.083482
ACAACGC5600.023.5047023
TACATGG106450.023.4950942
CGCAGAG165350.023.391318
ACCTGGG36400.022.342383
ACATGGG109600.022.0034893
ATAACGC3700.021.5989153
Produced by FastQC (version 0.11.5)