FastQCFastQC Report
Thu 2 Jun 2016
DRR024998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249276
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107360.20452344285192856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88990.16952814064263338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.048.8668061
GTATCAA149250.040.2290881
ATCAACG178250.033.2585753
TCAACGC179250.032.992784
CAACGCA188450.031.330715
TATCAAC191850.031.025812
AACGCAG195300.030.4197316
ACGCAGA223000.026.3232277
CGCAGAG223950.025.9807198
GTACATG145050.024.9595431
TACATGG143800.023.6063882
GCAGAGT244000.023.479419
ACATGGG146000.022.3150833
GTGGTAT45350.022.187081
TGGTATC42950.021.7841742
GAGTACT157000.020.81823312-13
AGAGTAC218500.020.56258210-11
CAGAGTA238850.019.55835710-11
TATAACG4350.019.4551472
AGTACTT169450.018.90038912-13
Produced by FastQC (version 0.11.5)