FastQCFastQC Report
Thu 2 Jun 2016
DRR024998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249276
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118380.22551681412827218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105000.20002758475645022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67630.12883681482932122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.049.12851
GTATCAA149050.038.7903251
ATCAACG187050.030.4894733
TCAACGC188550.030.1116224
CAACGCA196600.028.9742935
TATCAAC199750.028.7417342
AACGCAG205750.027.890826
ACGCAGA235650.024.130727
CGCAGAG236000.023.89588
TGGTATC41800.023.8475422
GTGGTAT45550.023.547781
GTACATG140550.022.7270151
TACATGG142300.021.7753222
GCAGAGT256800.021.429619
ACATGGG142700.020.65813
AGAGTAC233450.020.53329510-11
ACAACGC8450.019.4741343
AGTACTT182650.019.11716812-13
GAGTACT170650.019.0019112-13
CAGAGTA252700.018.1973410-11
Produced by FastQC (version 0.11.5)