FastQCFastQC Report
Thu 2 Jun 2016
DRR024997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3863483
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92480.2393695015611561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78310.20269275159228084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.12033183528955607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.055.0512771
GTATCAA122150.041.6527631
ATCAACG148550.033.8981673
TCAACGC150450.033.498294
TATCAAC155800.032.263322
CAACGCA156400.032.2218135
AACGCAG162600.031.2477236
GTGGTAT30550.027.8597621
ACGCAGA181400.027.6191757
CGCAGAG182550.027.2392128
TGGTATC28300.026.9170442
GCAGAGT195350.024.7563769
GTACATG105800.022.5781361
TACATGG106100.021.1841622
GAGTACT134950.020.99818412-13
AGAGTAC182450.020.8009810-11
CAGAGTA194200.020.7403810-11
ACATGGG109350.020.2517033
TACCTGG30800.019.999512
AGTACTT143050.019.234312-13
Produced by FastQC (version 0.11.5)