FastQCFastQC Report
Thu 2 Jun 2016
DRR024997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3863483
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105050.2719049106725719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95730.2477816001778706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.15454966412431478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67250.057.0822831
GTATCAA125500.039.771791
GTGGTAT29850.032.308221
ATCAACG156050.031.3933373
TCAACGC158000.031.0549434
TATCAAC162450.030.5065982
TGGTATC29800.030.1363032
CAACGCA164350.029.9122665
AACGCAG171750.028.6778286
ACAACGC6250.024.8237533
ACGCAGA196900.024.7983867
CGCAGAG196650.024.5909358
GCAGAGT209850.022.641019
GTACATG99850.021.5313681
GTACCTG33550.020.6123891
AGAGTAC197950.020.22729110-11
TACATGG102200.020.0589622
TACCTGG32350.020.057542
GAGTACT150700.019.89578212-13
CAGAGTA209350.019.8217210-11
Produced by FastQC (version 0.11.5)