FastQCFastQC Report
Thu 2 Jun 2016
DRR024996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419786
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94890.27747350272794846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77400.22632995164024883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47750.1396286200364584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.038.7898861
GTATCAA125250.034.8747521
TACCTGG35900.033.7888152
GTACCTG37300.033.0267331
ACAACGC7350.030.0634753
ATCAACG148750.029.0144523
TCAACGC149700.028.922854
TATAACG3850.028.0876142
TATCAAC155450.027.7651272
CAACGCA156800.027.7610865
AACGCAG163450.026.8002596
GTACATG85000.025.8327771
ACCTGGG46850.025.28833
TACATGG86250.024.094112
ACGCAGA181900.023.7704667
CGCAGAG181100.023.6415628
TACAACG9350.023.6338212
TCTGTCG11950.023.597158
TAACGCA5250.023.281844
GTCTCGC11800.023.1110211
Produced by FastQC (version 0.11.5)