FastQCFastQC Report
Thu 2 Jun 2016
DRR024996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419786
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106980.31282659207330515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91150.26653714589158506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63580.18591806621817858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82750.041.4391751
ACAACGC5100.038.7153243
GTACCTG32700.036.24971
TACCTGG33400.036.032742
ATAACGC4500.035.5197753
GTATCAA135300.035.0440521
TATAACG4600.031.681642
TAACGCA4950.030.3854734
ATCAACG164300.028.555963
TCAACGC165400.028.3038184
TACAACG7150.028.272632
TATCAAC170450.027.9393462
CAACGCA170950.027.3024375
AACGCAG178300.026.2824026
TCTGTCG12000.025.847998
GTACATG84350.024.5926361
ACGCAGA199350.023.2686567
CGCAGAG197900.023.2488238
TACATGG86450.022.839582
ACCTGGG48950.022.7614273
Produced by FastQC (version 0.11.5)