FastQCFastQC Report
Thu 2 Jun 2016
DRR024995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177512
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146610.2831668956054568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121850.23534469838022587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72380.1397968754104288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.053.7817461
GTATCAA182050.041.947421
ATCAACG223250.033.8665433
TCAACGC225650.033.5262074
TATCAAC229850.032.9570472
CAACGCA241450.031.484765
AACGCAG252900.030.1824236
ACGCAGA282500.026.6188347
GTGGTAT44100.026.5503751
CGCAGAG283050.026.2679778
GTACATG202650.024.6194671
TGGTATC46700.024.165272
GCAGAGT301650.023.8225089
TACATGG201650.023.7614652
ACATGGG209500.021.9049423
TACCTGG41250.021.3164522
TATACCG4700.021.0057895
GAGTACT182950.020.88392412-13
CAGAGTA293150.020.42503710-11
AGAGTAC279350.020.08810410-11
Produced by FastQC (version 0.11.5)