FastQCFastQC Report
Thu 2 Jun 2016
DRR024995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5177512
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169930.3282078341875403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146430.2828192382750634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97980.18924147351082912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103050.054.2325481
GTATCAA183850.040.300321
ATCAACG238650.030.6716773
TCAACGC243550.030.1435344
TATCAAC245800.029.9153842
CAACGCA254100.028.9844865
GTGGTAT47050.028.8954721
AACGCAG265200.027.8768636
TGGTATC48300.027.644642
ACGCAGA304150.024.1352927
GTACATG203900.024.0173471
CGCAGAG304750.023.9641748
TACATGG205850.022.862462
GCAGAGT323550.022.1507199
ACATGGG211700.021.385323
AGAGTAC299150.020.35988610-11
GAGTACT202300.020.04814712-13
TACCTGG43250.019.5670832
CAGAGTA314250.019.49373610-11
AGTACTT207500.019.17203512-13
Produced by FastQC (version 0.11.5)