FastQCFastQC Report
Thu 2 Jun 2016
DRR024994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4399969
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161030.3659798512216791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134890.30657034174558956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81570.18538766977676432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93050.057.0954441
GTATCAA180950.043.130941
ATCAACG225600.034.15583
TCAACGC228200.033.7235224
TATCAAC232650.033.2828142
CAACGCA241550.031.8586035
GTGGTAT39500.031.660951
AACGCAG251600.030.7309656
TGGTATC40100.029.8968452
ACGCAGA282350.027.1667987
CGCAGAG280800.027.032218
GTACATG206250.026.032241
TACATGG204700.025.5627042
GCAGAGT298350.024.8743829
ACATGGG213300.023.5399193
TACCTGG38900.023.3258362
TATACCG3550.022.5115645
GAGTACT185250.021.48742712-13
GTACCTG43850.021.2292671
CAGAGTA293350.020.8843610-11
Produced by FastQC (version 0.11.5)