FastQCFastQC Report
Thu 2 Jun 2016
DRR024994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4399969
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175370.3985709899319745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152620.3468660801928377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98860.2246834011785083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.057.2897831
GTATCAA181800.041.2705731
GTGGTAT40550.032.713871
ATCAACG233800.031.7321243
TCAACGC236500.031.2420544
TATCAAC244400.030.64582
CAACGCA246850.030.008295
TGGTATC43050.029.5973322
AACGCAG258000.028.783316
GTACATG201750.025.857811
ACGCAGA294050.025.0287657
TACATGG201800.024.9997082
CGCAGAG293050.024.9056878
ACATGGG206950.023.1269823
GCAGAGT312150.022.930079
ACAACGC9650.022.4113043
TATAACG4000.022.333152
GAGTACT199750.020.97506712-13
AGAGTAC296550.020.22980510-11
AGTACTT205100.020.11859712-13
Produced by FastQC (version 0.11.5)