FastQCFastQC Report
Thu 2 Jun 2016
DRR024993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117450.3483778997009794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97300.2886093626301004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.17461904602296002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.053.3772741
GTATCAA123250.041.1622121
ATCAACG152150.032.78553
TCAACGC153500.032.5253754
TACCTGG35600.032.3578952
TATCAAC158950.031.5326822
CAACGCA160800.031.134195
GTACCTG38950.030.6612931
AACGCAG166200.030.2604396
GTACATG132050.027.4879971
GTGGTAT29800.027.4534661
ACGCAGA188250.026.5895717
TACATGG133000.026.5139142
CGCAGAG188450.026.336898
ACATGGG136600.025.2973523
ACCTGGG43200.025.0312523
TGGTATC31550.024.8875032
GCAGAGT200100.024.169359
TACAACG6900.021.8054522
CAGAGTA195400.020.99840710-11
Produced by FastQC (version 0.11.5)