FastQCFastQC Report
Thu 2 Jun 2016
DRR024993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371339
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132460.39290026900290953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112500.33369530622699173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79850.23684951290866924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.054.8724671
GTATCAA120700.040.2100451
TACCTGG35250.035.338582
GTACCTG41300.031.0867061
GTGGTAT27900.030.6781621
ATCAACG159050.030.1454943
TCAACGC161200.029.7681754
TATCAAC163900.029.2538872
CAACGCA168100.028.5183225
ACCTGGG44050.028.1717553
AACGCAG174400.027.6224636
TGGTATC31200.026.6674212
GTACATG124900.026.0182291
TACATGG124450.024.8916022
ACGCAGA200000.023.9447297
CGCAGAG201400.023.6145868
ACATGGG130950.023.2608413
GTACCGG3350.022.4614261
GCAGAGT214950.021.6231089
ACAACGC7200.021.5445463
Produced by FastQC (version 0.11.5)