FastQCFastQC Report
Thu 2 Jun 2016
DRR024992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347826
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108840.2503320050066401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95730.2201790044035801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53230.12242900244858006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77000.050.7409061
GTATCAA134200.039.063421
TCAACGC162950.031.879094
ATCAACG163700.031.7348583
CAACGCA170600.030.5304875
TATCAAC170700.030.2957782
AACGCAG178150.029.3648246
ACGCAGA200600.025.7027027
CGCAGAG202700.025.3196078
GTACATG128750.024.7588461
GTGGTAT39100.023.6884921
TACATGG127950.023.0382882
GCAGAGT214450.023.0337569
TACCTGG31550.022.6499462
TGGTATC38500.022.3466952
GTACCGT2406.706614E-921.5426676
ACATGGG132300.021.2857973
GAGTACT139000.021.08104912-13
CAGAGTA210900.020.24575410-11
AGAGTAC197900.020.06764810-11
Produced by FastQC (version 0.11.5)