FastQCFastQC Report
Thu 2 Jun 2016
DRR024992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4347826
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114970.2644310052886201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103670.2384410047688201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64600.14858000297160004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.052.13071
GTATCAA137050.037.8990251
ATCAACG171300.029.8613643
TCAACGC172250.029.7440744
CAACGCA177800.028.868495
TATCAAC180750.028.431512
AACGCAG187300.027.504016
GTGGTAT35850.027.1655921
TGGTATC37950.025.3980392
GTACATG128650.025.1969181
ACGCAGA211650.024.2250547
CGCAGAG212850.023.8676858
TACATGG131050.022.9256632
GCAGAGT229150.021.637199
ACACCGT6750.021.5864456
ACATGGG135050.021.3752843
GAGTACT154950.020.15448212-13
AGAGTAC209700.019.84533510-11
CAGAGTA224650.019.56020710-11
AGTACTT158400.019.03310812-13
Produced by FastQC (version 0.11.5)