FastQCFastQC Report
Thu 2 Jun 2016
DRR024991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6679320
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119590.17904517226304476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96560.1445656144637478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95100.052.4622841
GTATCAA178950.041.6495131
ATCAACG215850.034.0906753
TCAACGC216900.033.9225964
CAACGCA232550.031.5976035
TATCAAC234100.031.5152572
AACGCAG241850.030.5705366
ACGCAGA267350.027.3539857
CGCAGAG268500.027.0261658
GTACATG194050.026.2680591
GTGGTAT48650.025.2272551
TACATGG191350.024.8440272
TGGTATC46950.024.3371662
GCAGAGT289050.024.2590629
ACATGGG197150.022.7761273
AGAGTAC269400.020.75128710-11
GAGTACT186050.020.32078212-13
CAGAGTA288200.019.9928510-11
AGTACTT197250.018.76193212-13
CATGGGG112400.018.4880124
Produced by FastQC (version 0.11.5)