FastQCFastQC Report
Thu 2 Jun 2016
DRR024991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6679320
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133710.20018504877742047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113500.1699274776474252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74660.11177784564895828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.051.721421
GTATCAA186200.041.4089431
ATCAACG236600.031.9927983
TCAACGC239150.031.5425034
TATCAAC250950.030.258172
CAACGCA250950.030.1155175
AACGCAG259950.029.1994176
ACGCAGA291050.025.7221437
CGCAGAG293600.025.402518
GTGGTAT52650.024.1246051
GTACATG197450.023.277281
TGGTATC50450.023.0193562
GCAGAGT320800.022.72139
TACATGG192950.021.9308322
AGAGTAC295700.020.9548510-11
ACATGGG197400.020.340043
AGTACTT218550.019.71859212-13
GAGTACT206050.019.63757712-13
CAGAGTA313850.018.83710910-11
GTACTTT228800.017.56180214-15
Produced by FastQC (version 0.11.5)