FastQCFastQC Report
Thu 2 Jun 2016
DRR024990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937139
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89900.30608016849049363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72220.2458855369119405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51190.17428524833179498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.051.084991
TACCTGG28800.042.1258132
GTATCAA115650.041.597371
GTACCTG31350.039.900651
ACCTGGG35100.033.8931773
ATCAACG145900.032.8733373
TCAACGC147950.032.4156344
CAACGCA154000.031.2610915
TATCAAC152900.031.2469272
GTACCCG2300.030.6689781
ACAACGC6750.030.651223
AACGCAG162200.030.0518676
GTACCGG3350.028.0750851
TATAACG3100.027.3043542
ACGCAGA180300.026.772037
CGCAGAG180600.026.3887848
GTACATG125000.025.5443971
TACATGG120250.024.558152
GCAGAGT190800.024.0420049
GTGGTAT30100.023.9034631
Produced by FastQC (version 0.11.5)