FastQCFastQC Report
Thu 2 Jun 2016
DRR024990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937139
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97510.33198973558963335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80290.27336125392771676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.19845843182770714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61150.052.3148271
GTATCAA119300.043.5352481
GTACCGG3500.041.668321
TACCTGG29550.039.2993622
ACAACGC7400.036.213823
GTACCTG33300.035.4602551
ATCAACG154050.033.235133
TCAACGC157400.032.5194664
TATCAAC162450.031.8070952
ACCTGGG35600.031.4309963
CAACGCA164800.031.40155
GTACCCG1859.094947E-1230.5155961
AACGCAG172450.030.1442746
ACGCAGA195150.026.466127
CGCAGAG195700.026.2947858
TATAACG3600.024.8140182
GCAGAGT205500.024.7664059
GTACATG126800.024.4499821
TACATGG123100.023.5271152
ATAACGC4600.023.5072173
Produced by FastQC (version 0.11.5)