FastQCFastQC Report
Thu 2 Jun 2016
DRR024989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221200
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85150.20171989007865063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.1704965412678859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63750.050.1500781
GTATCAA116200.040.5014721
ATCAACG136850.033.699293
TCAACGC139250.033.2523354
CAACGCA146050.031.8946045
TATCAAC147650.031.426852
AACGCAG152650.030.5753756
ACGCAGA172700.026.506987
CGCAGAG172500.026.3197448
GTACATG118900.024.5161741
GCAGAGT183300.024.1276869
TACATGG119950.022.967542
GTGGTAT32550.022.9661921
ACATGGG123250.021.5505353
GTACCGG4200.021.2690051
GAGTACT118500.021.0186512-13
TGGTATC34450.020.0606612
AGAGTAC170950.020.05601710-11
CAGAGTA183400.020.02710310-11
GTACTTT129200.019.00604414-15
Produced by FastQC (version 0.11.5)