FastQCFastQC Report
Thu 2 Jun 2016
DRR024989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221200
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89080.2110300388515114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79370.18802710129820904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.11570169620013265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63650.055.352031
GTATCAA118100.040.5858271
ATCAACG143750.032.638553
TCAACGC145400.032.228584
CAACGCA151650.030.9003355
TATCAAC153000.030.8507672
AACGCAG158350.029.740966
GTGGTAT29950.025.6000561
ACGCAGA182200.025.5355877
CGCAGAG181450.025.3821338
GTACATG118000.024.5555041
TACATGG118350.023.3181172
GCAGAGT195600.023.0173689
TGGTATC33100.022.7256132
ACATGGG118600.022.1978843
GAGTACT132600.020.18409212-13
AGAGTAC180150.020.17832210-11
ATAACGC4200.020.1479783
CAGAGTA191950.019.91720810-11
AGTACTT139750.018.71424712-13
Produced by FastQC (version 0.11.5)