FastQCFastQC Report
Thu 2 Jun 2016
DRR024988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417690
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88320.19992348942546898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76040.1721261564301705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.10179528214972078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.053.3193131
GTATCAA135550.041.071181
ATCAACG164750.033.275053
TCAACGC166150.033.0214774
TATCAAC173650.031.733512
CAACGCA174200.031.4656735
AACGCAG181550.030.3430336
ACGCAGA205050.026.4517257
CGCAGAG203450.026.4515328
GTGGTAT36850.025.5198121
GTACATG150550.025.0170881
TGGTATC36600.024.4083162
TACATGG151100.024.0536142
GCAGAGT222500.023.4052229
ACATGGG153900.022.7289813
GAGTACT130500.020.77803212-13
AGAGTAC200800.020.45741710-11
CAGAGTA215850.020.25041610-11
TACCTGG34250.019.9054832
CATGGGG82700.019.2717064
Produced by FastQC (version 0.11.5)