FastQCFastQC Report
Thu 2 Jun 2016
DRR024988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417690
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100380.22722282459837606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.2015533004805679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.1295699788803651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77550.053.807671
GTATCAA140800.040.160971
ATCAACG174150.031.8311023
TCAACGC175850.031.458154
TATCAAC181000.030.8089772
CAACGCA182100.030.2752065
AACGCAG193800.028.6889926
GTGGTAT34550.027.2322981
TGGTATC36000.024.8154562
ACGCAGA223250.024.5452127
CGCAGAG221900.024.4612968
GTACATG150050.023.6081181
TACATGG152550.022.1609212
GCAGAGT241400.022.0181169
ACATGGG154650.020.917023
TATAACG4700.020.0079822
ACAACGC7300.019.966423
GAGTACT154150.019.9232112-13
AGAGTAC224800.019.67198210-11
CAGAGTA236150.019.29366310-11
Produced by FastQC (version 0.11.5)