FastQCFastQC Report
Thu 2 Jun 2016
DRR024987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134730.434498111627928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113050.3645810993805185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.23674390540047643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81750.053.7196351
GTATCAA144950.041.001791
ATCAACG188600.031.4588323
TCAACGC190900.031.126054
TATCAAC191750.030.9195142
CAACGCA200650.029.7751715
AACGCAG205700.029.1322966
TAACGCA2950.028.683584
GTGGTAT35950.026.5504021
ACGCAGA228750.025.9292567
TATAACG3100.025.7833982
TACCTGG26350.025.6941812
CGCAGAG230850.025.4490748
GTACATG164400.025.1969531
TACATGG165400.024.4464552
GCAGAGT239000.023.6373279
TGGTATC39050.023.2374882
ACATGGG169600.022.8138683
TACAACG5900.022.3130512
GAGTACT144950.020.83141312-13
Produced by FastQC (version 0.11.5)