FastQCFastQC Report
Thu 2 Jun 2016
DRR024987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153810.49603024233275145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131180.4230495233678586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92830.29937252061471503No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35050.1130346531029383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.053.3498881
GTATCAA148900.040.7167551
ATCAACG196200.030.81393
TCAACGC198850.030.4653364
TATCAAC201800.029.9835552
CAACGCA207600.029.2039095
AACGCAG214400.028.2995856
TATAACG2850.028.0433442
TACCTGG25250.026.4394682
ACGCAGA243850.024.6304727
GTGGTAT35600.024.3098751
CGCAGAG246650.024.2937018
GTACATG169700.023.9467641
TACATGG170300.023.4931162
GCAGAGT258200.022.8611479
ACATGGG174450.022.2055193
TACAACG6750.021.5915412
GTACCTG32800.021.2228051
AGTACTT156900.020.8926212-13
GAGTACT157300.020.8544312-13
Produced by FastQC (version 0.11.5)