FastQCFastQC Report
Thu 2 Jun 2016
DRR024986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688820
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114070.2432808254528858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97290.2074935698107413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.12098992923592718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90150.046.777281
GTATCAA161700.039.3946761
ATCAACG197900.031.808153
TCAACGC199600.031.5133484
TATCAAC209000.030.1866172
CAACGCA211000.029.8759785
AACGCAG219750.028.7679566
GTACATG180650.027.4030321
TACATGG178600.026.6120282
ACGCAGA243100.025.5197727
CGCAGAG244150.025.2945218
ACATGGG184150.025.2225423
GTGGTAT44950.023.9504681
GCAGAGT260700.022.7332699
TACCTGG35650.021.7586862
TGGTATC48950.021.2250062
CATGGGG99850.020.5746444
AGAGTAC232300.020.30280310-11
GAGTACT155850.020.14357812-13
GAGTACA134150.020.1154371
Produced by FastQC (version 0.11.5)