FastQCFastQC Report
Thu 2 Jun 2016
DRR024986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688820
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113420.24189454916162273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97560.20806940765480442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64850.1383077192129363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93700.047.5373421
GTATCAA164450.037.9257471
ATCAACG206100.030.0615753
TCAACGC207950.029.7880974
TATCAAC214250.028.962232
CAACGCA217200.028.5627755
AACGCAG227150.027.3734026
GTACATG187050.025.2967551
TACATGG185700.024.3777332
ACGCAGA254000.024.2009567
CGCAGAG254600.023.9408768
ACATGGG190500.022.9242463
GCAGAGT273050.021.8242939
GTGGTAT48350.021.2072581
AGAGTAC244250.020.28967710-11
GAGTACT157650.019.19795812-13
AGTACTT164950.019.13184412-13
CAGAGTA267850.018.66865710-11
TGGTATC51150.018.4726542
CATGGGG106000.018.4004544
Produced by FastQC (version 0.11.5)