FastQCFastQC Report
Thu 2 Jun 2016
DRR024985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219786
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.27824830594331423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73920.2295804752241298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.1334250164451923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.047.0684051
GTATCAA101350.039.9969061
ATCAACG119400.033.4353453
TCAACGC120200.033.3291324
CAACGCA125600.031.8917335
TATCAAC126450.031.8334982
AACGCAG132200.030.3996356
TACCTGG26450.027.2016472
ACGCAGA147900.026.979857
GTACATG91000.026.9239791
CGCAGAG148650.026.5903688
TACATGG89450.025.8125292
GTACCTG29050.025.4153671
GCAGAGT157900.024.4961329
ACATGGG92400.024.2744183
GTGGTAT29400.024.1530781
TGGTATC30200.022.4225482
GAGTACT108550.021.12642112-13
CATGGGG55050.021.097384
CAGAGTA155200.020.41023310-11
Produced by FastQC (version 0.11.5)