FastQCFastQC Report
Thu 2 Jun 2016
DRR024985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219786
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101020.313747559620422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87610.27209882892838216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57260.17783790599747934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.052.254651
GTATCAA109350.038.54741
ATCAACG133150.031.3575043
TCAACGC134300.031.0428874
CAACGCA139400.030.0757585
TATCAAC141950.029.5805322
AACGCAG146000.028.844946
TACCTGG27350.027.5075912
GTACCTG29400.026.4023481
CGCAGAG167350.024.8802688
ACGCAGA168500.024.8224077
GTGGTAT30500.024.5246721
TATAACG3650.024.4765762
GTACATG92200.024.38951
ATAACGC3700.024.1446863
ACAACGC5900.023.1108363
TACATGG92600.022.7994982
TGGTATC31200.022.7568572
GCAGAGT180400.022.7417989
GTACCGT1452.5343556E-522.6905236
Produced by FastQC (version 0.11.5)