FastQCFastQC Report
Thu 2 Jun 2016
DRR024984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360294
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96160.28616543671476363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78830.23459256838836126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.14590985193557468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.051.5391041
GTATCAA114950.040.654211
ATCAACG137450.033.3137053
TCAACGC139700.032.8434834
TATCAAC143400.032.2935072
TATAACG3100.031.848262
CAACGCA146000.031.4883175
AACGCAG150950.030.6403056
ACAACGC6350.029.6138553
GTGGTAT29150.027.9020211
ACGCAGA166250.027.536937
TACAACG6800.026.9639662
CGCAGAG168700.026.9419848
TACCTGG32150.026.9069792
GTACCTG35250.026.141171
ATAACGC4150.024.9220243
GCAGAGT178450.024.6267329
GTACATG99950.024.3654861
TGGTATC33100.023.862142
TACATGG99350.022.5251242
Produced by FastQC (version 0.11.5)