FastQCFastQC Report
Thu 2 Jun 2016
DRR024984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360294
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108950.32422758246748645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93590.27851729640323136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63350.18852517071422917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63400.050.5957571
GTATCAA115750.040.756691
ATCAACG148600.031.256283
TCAACGC150800.030.7924964
TATCAAC152950.030.5852152
CAACGCA155550.029.8521885
ACAACGC5100.029.4970723
AACGCAG162800.028.7537296
TACCTGG29400.026.8658072
TAACGCA4350.025.9305214
ACGCAGA184650.025.1966517
GTACCTG33450.024.8883511
CGCAGAG186350.024.8154778
GTGGTAT31300.024.7946851
ATAACGC4600.023.5054823
TGGTATC32450.023.181592
GCAGAGT197050.022.8717359
GTACATG96950.022.7047671
TATAACG4800.022.52812
TACAACG5950.022.1247832
Produced by FastQC (version 0.11.5)