FastQCFastQC Report
Thu 2 Jun 2016
DRR024983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90860.36839745081614267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75120.304578654031572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.18999674824173945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.054.892841
TACCTGG27650.046.2494282
GTATCAA90050.041.455961
GTACCTG32250.040.820521
ACCTGGG33850.036.1094473
TATAACG2500.035.7310832
GTGGTAT20900.035.0936471
GTACCGG3250.034.7198681
TGGTATC21100.032.754242
ATCAACG113300.032.447633
ACAACGC6250.032.3440673
TCAACGC114850.032.007784
TATCAAC119400.030.6342932
CAACGCA120000.030.5930755
AACGCAG126300.029.2135546
ACGCAGA139550.026.1692717
GTACATG88400.026.167551
CGCAGAG140400.025.9098878
GTACCCG2208.731149E-1125.6453591
TAACGCA3850.025.6411974
Produced by FastQC (version 0.11.5)