FastQCFastQC Report
Thu 2 Jun 2016
DRR024983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100800.40869979135226925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84560.34285371385662583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.24153022391720907No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.10083694256875929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2700.053.979622
GGTATCA49100.052.8843771
TACCTGG28150.045.7619062
GTATCAA93650.042.193091
GTACCTG32600.041.268461
ACAACGC5150.040.166113
TAACGCA4000.039.9518054
GTACCGG2950.039.864631
ACCTGGG34100.036.3968543
GTGGTAT20950.033.6803861
ATCAACG121050.032.3515053
TCAACGC123150.031.8309124
CAACGCA128250.030.601775
TATCAAC129950.030.3541152
TGGTATC22450.029.7374632
AACGCAG134050.029.452436
ATAACGC5800.028.3696753
GTATAAC7250.027.2509161
GTACATG88800.026.5396121
ACGCAGA151250.025.9135237
Produced by FastQC (version 0.11.5)