FastQCFastQC Report
Thu 2 Jun 2016
DRR024982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215739
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77070.23966497281029336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64320.200016232660673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.13178930255222826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.054.5416871
TACCTGG35250.041.884742
GTATCAA93150.040.180511
GTACCTG39500.037.6162261
ACAACGC6250.032.3474433
ATCAACG115250.032.1059343
TCAACGC117450.031.5431064
TATAACG3450.031.3468592
TATCAAC119650.031.0848712
CAACGCA123500.030.1853985
AACGCAG128350.029.1496686
GTACCGA1555.06825E-827.3021011
GTACCGG4150.027.1924551
ACCTGGG52400.027.0076453
ACGCAGA143950.025.6952277
TGGTATC24050.025.4163722
CGCAGAG144150.025.3335328
GTCTCGC10800.025.2516351
ATAACGC4350.024.859373
GTACATG81350.024.8539941
Produced by FastQC (version 0.11.5)