FastQCFastQC Report
Thu 2 Jun 2016
DRR024982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215739
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89120.27713691938307183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74530.23176632183146703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53620.16674238798608967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52350.055.2579771
TATAACG3450.044.9712942
GTATCAA97300.042.1541371
TACCTGG33650.041.775982
ATAACGC4150.040.782583
GTACCTG37050.037.832461
ACAACGC6700.033.6811333
TCTGTCG11300.032.0236248
ATCAACG126800.031.9971923
TCAACGC127800.031.6653674
GTGGTAT24250.030.8405361
TAACGCA4900.030.6948954
TATCAAC133200.030.6023962
CAACGCA134250.030.2840585
AACGCAG140700.028.995086
CTGTCGC13150.028.9479269
TGGTATC24150.028.61812
GTCTCGC10650.027.8245071
TACAACG8700.027.0203722
ACCTGGG51750.026.1638973
Produced by FastQC (version 0.11.5)