FastQCFastQC Report
Thu 2 Jun 2016
DRR024981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351329
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213090.6358373051407367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169810.5066945083577291No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115900.3458329516439598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67160.20039811071965777No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.1554607142420216No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.1327234658250503No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.10366036876713686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116850.048.446711
TACCTGG39150.043.4734232
GTATCAA205200.039.9380231
TACAACG8350.039.4147382
TATAACG4550.035.1329462
ACCTGGG46300.035.0331083
GTACCTG49550.034.7299351
ACAACGC9700.033.443983
ATCAACG260250.031.0907153
TCAACGC263950.030.6895984
TATCAAC266800.030.4159972
CAACGCA275300.029.4046275
AACGCAG284950.028.601676
GTACAAC13250.026.2592541
GTACATG217000.026.2175521
TACATGG213750.025.7791462
TAACGCA6400.025.7108084
ACGCAGA319450.025.3203377
CGCAGAG319850.025.1417318
ACATGGG217950.024.5054133
Produced by FastQC (version 0.11.5)