FastQCFastQC Report
Thu 2 Jun 2016
DRR024980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885830
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149850.38563189846184726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126890.32654542272822024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78840.20289101684839533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84600.056.5739671
GTATCAA154300.042.109621
ATCAACG194800.032.9906463
TCAACGC196900.032.5893634
TATCAAC199750.032.2218362
CAACGCA205400.031.2844855
TACCTGG36050.029.9955062
AACGCAG215700.029.9618596
GTGGTAT35250.029.7430131
TGGTATC36450.027.3446242
ACGCAGA240350.026.5750947
CGCAGAG242800.026.2098088
GTACATG167500.025.0935381
ACAACGC8900.024.8267063
GCAGAGT255350.024.3694659
TACAACG8500.024.3370192
TACATGG166250.024.0658662
GTACCTG47050.023.7824481
ACATGGG173300.022.5159783
ACCTGGG46400.022.2903023
Produced by FastQC (version 0.11.5)