FastQCFastQC Report
Thu 2 Jun 2016
DRR024980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885830
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169320.43573702400774095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145040.37325359060998553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101320.26074223524961204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39740.10226901331247121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.056.887611
GTATCAA155950.041.8033831
ATCAACG203000.031.9126633
GTGGTAT34550.031.7206021
TCAACGC206950.031.2735944
TACCTGG36800.030.915982
TATCAAC212000.030.6248042
CAACGCA215600.030.0624775
TGGTATC35350.029.3912682
AACGCAG224950.029.000616
ACAACGC9000.026.1178473
ACGCAGA257000.025.1622737
CGCAGAG259600.024.8013238
GTACCTG49200.024.0917871
GTACCGG5150.023.7465021
TATAACG4650.023.2536092
GTACATG159000.022.95611
GCAGAGT276550.022.9243629
ACCTGGG47400.022.811793
TACATGG158850.022.2855432
Produced by FastQC (version 0.11.5)