FastQCFastQC Report
Thu 2 Jun 2016
DRR024979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4678200
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122580.2620238553289727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104120.22256423410713522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.1322944722329101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79850.054.1710471
GTATCAA148600.040.5307461
ATCAACG186350.032.1162953
TCAACGC188100.031.841134
TATCAAC193800.030.9308682
CAACGCA196250.030.516535
AACGCAG205350.029.2059256
GTGGTAT37450.027.3690011
TACCTGG42150.026.212352
ACGCAGA229800.025.831237
CGCAGAG231550.025.5342458
GTACATG143200.025.4127831
GTACCTG44800.025.292591
TGGTATC38150.025.2635862
TACATGG136650.025.1159112
TATAACG4600.024.5295242
GCAGAGT246450.023.360919
ACATGGG143600.023.2123033
AGAGTAC231150.020.46455810-11
TAACGCA6950.020.292914
Produced by FastQC (version 0.11.5)