FastQCFastQC Report
Thu 2 Jun 2016
DRR024979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4678200
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138010.2950066264802702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121710.26016416570475825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.1690393741182506No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.053.3086281
GTATCAA156900.041.1954271
ATCAACG200150.031.9678693
TCAACGC201650.031.6053944
TATCAAC206750.031.040652
CAACGCA210400.030.3133455
AACGCAG221200.028.8545556
GTGGTAT38900.028.6605111
TGGTATC38250.027.9021782
ACGCAGA246200.025.7502757
CGCAGAG245950.025.508948
TATAACG4500.024.030292
GTACATG141100.023.9377121
TACATGG138250.023.2273272
GCAGAGT267350.023.098189
ACAACGC7600.022.2689673
ACATGGG137800.021.6979663
TACCTGG37550.021.0350252
AGAGTAC249450.020.85579310-11
TCTGTCG11500.020.8415648
Produced by FastQC (version 0.11.5)