FastQCFastQC Report
Thu 2 Jun 2016
DRR024978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.27245267760420283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75180.22685781705929745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46870.14143157602512998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61550.049.6469961
GTATCAA113050.040.2127341
TATAACG3000.034.474862
TACCTGG31900.033.6004682
ATCAACG139650.032.1809163
TCAACGC141150.031.838454
ACAACGC6800.031.800243
TATCAAC142900.031.4504452
CAACGCA147250.030.487125
GTACCTG36200.030.1291661
AACGCAG155000.028.958836
TACAACG7300.027.6915152
ACGCAGA170850.026.0781337
CGCAGAG170750.025.8456848
TCTGTCG10700.025.4757738
GTCTCGC10550.025.3997861
GTACATG89600.025.3948141
TACATGG90300.024.3125342
GCAGAGT180250.023.831659
ACATGGG87900.023.4243053
Produced by FastQC (version 0.11.5)