FastQCFastQC Report
Thu 2 Jun 2016
DRR024978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102860.31038301493375015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87650.2644864015063504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63110.19043624414222216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62950.049.6172031
TATAACG3000.045.44722
GTATCAA116200.040.8050881
ACAACGC7350.034.540113
TACAACG7550.033.003452
ATAACGC4050.032.5027663
TACCTGG33550.031.9501382
ATCAACG149050.031.3840073
TCAACGC150250.031.1253664
TATCAAC151450.030.9807342
CAACGCA156600.030.0133295
GTACCTG36300.029.286111
TCTGTCG10050.028.9924988
AACGCAG165900.028.5845246
GTCTCGC10450.027.4582861
ACGCAGA184650.025.4004387
GTGGTAT31700.025.3744541
CGCAGAG184700.025.2408988
GTACATG85800.024.835381
CTGTCGC12300.024.0710749
Produced by FastQC (version 0.11.5)